rabbit polyclonal anti duox2 antibody  (Novus Biologicals)

Journal: Archives of biochemistry and biophysics

Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

doi: 10.1016/j.abb.2023.109787

Figure Lengend Snippet: Fig. 2. Expression of Nox-Duox in human villous cytotrophoblast using RT-PCR and Sanger sequencing. (A) PCR products of cDNA from term placenta VCT (100 ng/well). Primers used targeted Nox1, Nox2, Nox3, Nox4, Nox5, Duox1 or Duox2. Revelation is performed using UV light and Minibis transilluminator. (B) Sanger sequences obtained by Eurofins from PCR amplification products.

Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

Journal: Archives of biochemistry and biophysics

Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

doi: 10.1016/j.abb.2023.109787

Figure Lengend Snippet: Fig. 4. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5, anti-DUOX1 and anti-DUOX2 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 50 μm. On the right of the panel, magnification of term chorionic villi was done. Scale bar = 20 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis.

Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

Techniques: Immunostaining, Negative Control

Journal: Archives of biochemistry and biophysics

Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

doi: 10.1016/j.abb.2023.109787

Figure Lengend Snippet: Fig. 5. Expression of NOX family proteins during Pre-eclampsia (PE). Immunoblot using (A) NOX1-3-5 (B) NOX4 and DUOX1 antibodies is performed on physiological term placentas (control, n = 10) and pre-eclamptic placentas (EARLY-PE < 34GW, n = 11 and LATE-PE > 34 GW, n = 7). Vinculin is used as loading control. Graphs represent the total amount of each protein relative to Vinculin level determined by quantification of immunoblot using ImageStudiolight from LICOR. The mean is reflected by long horizontal lines and standard deviations by short horizontal lines. Statistical analysis was performed using Mann-Whitney test. *p < 0,05.

Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

Techniques: Expressing, Western Blot, Control, MANN-WHITNEY

Journal: Archives of biochemistry and biophysics

Article Title: Placental cartography of NADPH oxidase (NOX) family proteins: Involvement in the pathophysiology of preeclampsia.

doi: 10.1016/j.abb.2023.109787

Figure Lengend Snippet: Fig. 6. Immunolocalization of NOX family proteins during pregnancy. Chorionic villi are fixed in 4% PFA and included in Paraffin. Immunostaining of NOX is revealed with Biotin-Streptavidin-DAB system. Antibodies anti-NOX1, anti- NOX3, anti-NOX4, anti-NOX5 and anti-DUOX1 are used to immunostain. Non-specific IgG is performed as negative control (small box top right of each image). Scale bar = 20 μm. On the right of the panel, magnification of term chorionic villi is done. Scale bar = 10 μm. Experiments are reproduced 3 times per group. VCT: cytotrophoblast, ST: Syncytiotrophoblast, V: Vessels, MA: Mesenchymal axis

Article Snippet: Anti-NOX1 rabbit antibody (# SAB4200097) was from Sigma, anti-NOX2 mouse monoclonal antibody (#41012), anti-NOX3 rabbit polyclonal antibody (# 41292), anti-DUOX1 rabbit polyclonal antibody (# 16232), anti-DUOX2 rabbit polyclonal antibody (# 61576SS) were from Novus Biologicals, anti-NOX4 rabbit polyclonal antibody (# ABC459) was from Merck, and anti-NOX5 rabbit polyclonal antibody (# ab191010) was from Abcam.

Techniques: Immunostaining, Negative Control

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Pedigrees and variants of 3 unrelated families carrying DUOX2 variants. Pedigrees of family 1(A), 2(B), and 3(C). The genotype and phenotype information are displayed, +/− represents a heterozygous nonsense (c.1588A>T; p.K530X) variant in the DUOX2 gene, and −/− represents the wild type. (D) The four DUOX2 variants in these 3 families were validated by Sanger sequencing. Their locations are depicted in a corresponding lollipop plot of DUOX2 (created with IBS) and the conserved domains of the hDuox2 protein are also shown (E).

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Variant Assay, Sequencing

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: The truncated protein, hDuox2 K530, is overexpressed in adenomas. (A) Immunohistochemistry (IHC) staining of hDuox2 with an antibody targeting the N-terminal 1–100 amino acids of human Duox2 protein in the adenoma and normal tissues from a DUOX2 p.K530X carrier. The expression of non-phospho (active) β-Catenin was also detected in these 2 samples by IHC analysis. (B) The positions of the amino acid sequence recognized by 2 different anti-Duox2 antibodies are marked. (C) Immunofluorescence staining of wild-type and truncated proteins of hDuox2 in adenomas and normal tissues from a DUOX2 p.K530X carrier as well as the tumor from a DUOX2 wild-type patient. AA, amino acid.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Immunohistochemistry, Expressing, Sequencing, Immunofluorescence, Staining

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Overexpression of truncated protein, hDuox2 K530, promotes cell proliferation through the unfolded protein response. (A) Construction of the RKO cell line with stable expression of hDuox2 K530. (B) Growth curve of RKO-hDuox2 K530 and the control group. **** P < 0.0001 (C) Confocal microscopy with anti-6 × His-tag (red) and anti-calreticulin (green) in the RKO-hDuox2 K530 cell line. (D) Immunohistochemistry staining (100×) of GRP78 in the adenoma and normal tissues from a DUOX2 p.K530X carrier. EV, empty vector.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Over Expression, Expressing, Confocal Microscopy, Immunohistochemistry, Staining, Plasmid Preparation

Journal: Cancer Biology & Medicine

Article Title: A truncated protein product of the germline variant of the DUOX2 gene leads to adenomatous polyposis

doi: 10.20892/j.issn.2095-3941.2020.0305

Figure Lengend Snippet: Schematic diagram of truncated protein, hDuox2 K530, causing adenomatous polyposis. The diagram shows that the truncated protein, hDuox2 K530, is translated due to a nonsense variant (c.1588A>T) occurring in the DUOX2 gene. Therefore, this misfolded protein is retained in the endoplasmic reticulum and activates an unfolded protein response, which could induce abnormal cell proliferation.

Article Snippet: The slides were incubated simultaneously with rabbit anti-DUOX2 (400–500 amino acids; 1:500 dilution; NB110-61576; Novus Biological, Centennial, CO, USA) and mouse anti-DUOX2 (628–680 amino acids; 1:500 dilution, sc-398681; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Variant Assay